Alphaglucosidase inhibitors agis are oral antidiabetic drugs used for diabetes mellitus type 2 that work by preventing the digestion of carbohydrates such as starch and table sugar. The yeast alpha glucosidase was dissolved in 100 mm phosphate buffer ph 6. In vitro studies on alpha amylase and alpha glucosidase. Lamiaceae from jordan by gc and gcms and to report the actual composition of their fresh leaves and flowers using spme solid phase micro extraction. Asclepiadaceae has been known to posses potential antidiabetic activity, and the gymnemic acids were reported as the main bioactive components in this plant species. Optimum water extraction conditions for phenolics of pomegranate peels were investigated by fractional factorial and facecentered central composite designs. On hydrolysis of the oligosaccharide by endoacting. Gras notice 703, alphaglucosidase from aspergillus niger. Glucosidase hydrolyzes carbohydrates by acting on terminal, nonreducing 1 4linked dglucose residues with the release of dglucose. Alpha amylase, alpha glucosidase and glycation inhibitory. Alpha amylase and alpha glucosidase inhibitory effects of. Dglucopyranoside, acarbose, bakers yeast alpha glucosidase were purchased from sigma usa. Diabetes mellitus is a metabolic disorder of glucose metabolism.
Pdf antidiabetic activity and phytochemical screening of. Colorimetric ab174093 activity assay kit alphaglucosidase. Purification of alphaglucosidase inhibitors from grape. Alphaamylase from kerry ingredients, ireland a dilution of the. Alphaglucosidase assay of extracts was carried out according to method of bachhawat et al with slight modification. The difference in the physicochemical properties of polysaccharides. The management of blood glucose level is the hallmark in the treatment of this disease. After eliminating statistically unimportant factors, a face. Antidiabetic antioxidant and phytochemical profile of. Glucosidase inhibitory activity of dichloromethane. The alphaglucosidase inhibition was assayed using purified enzyme according to the modified method described by matsui et al. The alphaglucosidase inhibitors are contraindicated in patients with diabetic ketoacidosis, inflammatory bowel disease, ulcerative colitis, intestinal obstruction, any chronic intestinal disease disrupting digestionabsorption, any condition that may deteriorate as a result of. The purpose of the present study was to investigate the inhibitory effect of morinda lucida leaf. Inhibitory effects of puerh tea on alpha glucosidase and.
Glucosidase inhibitory assay plant extracts were evaluated for. Alphaglucosidase inhibitory activity of garcinia lateriflora. However, the specific components responsible for the hypoglycemic effect still remain unknown. However, further investigation of the plant, including identification.
Determination of yeast alpha glucosidase inhibitor activity pnitrophenyl. These products include alphaamylase and glucosidase inhibitors. Carbohydrates are normally converted into simple sugars monosaccharides by alphaglucosidase enzymes present on cells lining the intestine, enabling monosaccharides to be absorbed through the intestine. The objective of this project was to determine the optimum procedures for extracting total phenols tp, total flavonoids tf and.
Puerh tea was presumed to have antihyperglycemic effects via inhibition on alphaamylase and alphaglucosidase. Dichloromethane extract was the most potent, with inhibitory activity of 97. Pdf isolation and identification of alphaglucosidase inhibitors. Isolation and identification of alphaglucosidase inhibitors from the stem bark of the nutgall tree rhus javanica linne. Phytochemical screening was carried out on the stem extracts using different. Glucosidase inhibitory activity of selected malaysian plants ncbi. Inhibition of the alpha glucosidase by various solvent extracts of. One minute for extraction time was enough to extract the phenolics from pomegranate peels. Fifteen chemical constituents were found by gcms analysis.
The aglucosidase enzyme preparation is intended for the hydrolysis of terminal, nonreducing 14linked alphadglucose residues with release of alphadglucose used in the fermentation. Black beans contain high phenolic contents that are considered potent antioxidants. Glucosidase inhibitory assay the effect of the plant extracts on. D i a b e tes o f mt l ab n r u o siol journal of diabetes. The results of the both enzyme inhibition activity was found in a dosedependent manner, the strongest activity in case of. The aqueous stem extract was selected from other extracts ethanol, acetone, petroleum ether and chloroform for the in vitro study of antidiabetic activity by alpha. Cinnamomum zeylanicum, a spice, has been used in traditional medicine for. In the same way, ebe7 fraction was the most active against.
The a glucosidase enzyme preparation is intended for the hydrolysis of terminal, nonreducing 14linked alpha dglucose residues with release of alpha dglucose used in the fermentation. Multistep optimization of glucosidase extraction from. Alphaamylase and alphaglucosidase enzyme inhibition and antioxidant potential of 3oxolupenal and katononic acid isolated from nuxia oppositifolia. Polar solvents help in the extraction of hydrophilic compounds and nonpolar solvents aids in the extraction of lipophilic compounds 15, 16. Moreover, mo and bsl have improved insulin secretion, in vivo antioxidant catalase, lipid peroxidation, acute and subchronic bgl, and normalized alphaglucosidase and hba1c levels. Glucosidase is an intestinal enzyme that catalyzes the break of. Where y is ic 50 50, m is the gradient of the curve, x is the concentration and c is the intercept at y axis 2. In the present study, the in vitro study revealed that the extract of g. Therefore, agis have been used as antidiabetic drugs to delay the breakdown of carbohydrate in the gut, and consequently slow the absorption of sugars, which will consequently lower postprandial insulin levels 10. This may be achieved through the use of oral hypoglycemic drugs such as biguanides, insulin secretagogues, and. Glucosidase hydrolyzes the substrate mix to release the pnitrophenol that can be measured colorimetrically od 410 nm. Alpha glucosidase inhibition by stem extract of tinospora. Alpha amylase and alpha glucosidase inhibitory effects of aqueous stem extract of salacia oblonga and its gcms analysis braz. At the concentration of 10mgml the plant showed appreciable alpha amylase and alpha glucosidase inhibitory activity 78.
This is an easy, quick and highthroughput capable kit that can measure 0. Relatively little is known about their ability to inhibit the carbohydratehydrolyzing enzymes amylase and glucosidase from releasing glucose from starch andor disaccharides. The ability of berry extracts to inhibit alphaglucosidase. Compared with acarbose, ebe7 fraction indicated obviously lower ic 50 values.
The alphaglucosidase inhibition assay was performed using a method from schmidt, lauridsen, dragsted, nielsen, and staerk 2012a. Hangju cmps, the response surface methodology was applied to optimize the parameters for extraction progress of cmps by ultrasound, with heat reflex extraction hre performed as the control. All solvents for extraction and isolation processes were purchased from thail oil co. Invitro alpha amylase and alpha glucosidase inhibition. Alphaamylases 100200 assays per kit aoac method 2002. The substrate solution pnitropheynyl glucopyranoside pnpg 3. Dglucopyranoside that is hydrolyzed specifically by. In a 96well microplate, each well was added with 10 l of extract dissolved in dmso and 90 l of 0. In abcam s alphaglucosidase activity assay kit colorimetric ab174093. Lineweaver burk plot for the activity of alphaglucosidase in the presence of varying concentrations of the terpenoid rich extract of m.
Alphaglucosidase inhibitory activity and phytochemical. It is also the major enzyme produced during the malting process. Metabolite profiling and inhibitory properties of leaf. The alpha glucosidase inhibition assay was performed using a method from schmidt, lauridsen, dragsted, nielsen, and staerk 2012a. Five potential factors were selected for the fractional factorial design. Glucosidase inhibitory activity of polyphenols from the. Isolation, purification and characterization of glucosidase from leaves of hordeum vulgare suman tiwari and o. Yet, alphaglucosidase inhibition for the acetone demonstrated the highest correlations with tp, tf. Alpha amylase and alpha glucosidase enzyme inhibition and antioxidant potential of 3oxolupenal and katononic acid isolated from nuxia oppositifolia.
Defects in glucosidase have been implicated in pompe disease and diabetes. The ic 50 values of stem extract was found to be 73. G5003, g66, g7256, g8889, g0660, and g3651 principle. Other drugs that belong to this class are miglitol and voglibose. Antioxidants free fulltext extraction optimization. Ccdbased optimization of extraction of active bglucosidase the effect of temperature x1 23, 25, 30, 35, and 37 c and ph x2 3. Alphaglucosidase from saccharomyces cerevisieae, paranitrophenylalphad glucopyranosidse and acarbose were obtained from sigmaaldrich, germany. Pphg is a major risk factor for diabetic vascular complications leading to disabilities and mortality in diabetics. The pancreatic alphaamylase in contrast hydrolyses. Pdf alpha amylase, alpha glucosidase and glycation.
355 1096 1548 45 1372 664 1318 165 1285 1031 1528 404 1119 592 1355 416 83 1326 903 1535 1547 1420 1127 4 574 388 273 1172 758 1179 1091